Pganps or aluminum adjuvant alum to jevlp significantly protected more than 90% of the mice. Pga is a naturally occurring biopolymer made from repeating units of lglutamic acid, dglutamic acid, or both. Author links open overlay panel dongxue ren a pengcheng chen a pu zheng a zhi. Safety and photochemotherapeutic application of poly. First, we assessed the eye irritancy potential of gamma pga in.
Pga nanoparticles nps with good photoacoustic and photothermal properties were prepared, and their chemodynamic performance based on a fentonlike reaction was explored due to its good catalytic effect. In this study nanogel was created from methacrylated poly gamma glutamic acid nanoparticles by visible dental blue light. These stable selfassembled nanoparticles were characterized by dynamic light scattering, transmission electron microscopy, and atomic force microscopy, which demonstrated that the nanosystem consists of spherical. Herein, we prepared hydrogels via dropwise addition of poly. These results will be helpful and informative in the manuscript, the authors showed a new contact lens care solution composed of poly gamma glutamic acid. Development and fabrication of novel proton exchange membranes pems with excellent performance have a great significance to the commercial application of pem fuel cell. The nanosystems were stable in aqueous media at low ph conditions. It is made of units of l and dglutamic acids connected with amides between. Poly c glutamic acid cpga is a naturally occurring biopolymer made up of repeating units of l glutamic acid, d glutamic acid or both. Pga is a polyanionic and multifunctional polymer biosynthesized by bacillus subtilis through fermentation, and is composed of repetitive glutamic acid units connected by.
Pga nanoparticles have been evaluated for use as antigen delivery carriers and delivery of influenza virus hemagglutin ha vaccine. Kr20080106482a cholesterolaminecontaining polygamma. Biomedical potential of ultrafine ag nanoparticles coated. The complex selfassembly behavior is investigated in detail. The solubility and size of these nanoparticles in the dried and swollen states will be described and discussed. Pga because it is biodegradable, biocompatible, nontoxic and nonimmunogenic. Full text raspberrylike polyyglutamic acid hydrogel. Soluble microbial products smps are of significant concern in the natural environment and in engineered systems. Poly gamma glutamic acid gamma pga is a natural, edible polypeptide in which glutamate is polymerized via gamma amide linkages. The synthesis and characterization of polygammaglutamic. Oct 27, 2016 okamoto s, matsuura m, akagi t, et al. Pga is a natural macromolecular polymer that is biodegradable, edible and nontoxic.
Poly gamm glutamic acid chitosan hydrogel nanoparticles for effective preservation and delivery of fermented herbal extract for enlarging hair bulb and enhancing hair growth hye su kim,1 hakyoung kwon,2 dong hoon lee,1 thao nguyen le,1 hyejin park,2 moon il kim1 1department of bionano technology, gachon university, seongnam, gyeonggi 120, republic of korea. This protein contains a gla domain at the nterminus, preceded by a propeptide sequence required for posttranslational gamma carboxylation of specific glutamic acid residues by a vitamin kdependent gamma carboxylase. Glutamic acid nanoparticles based visible lightcurable hydrogel for biomedical application article pdf available in journal of nanomaterials 20163. Copolymer nanoparticles prepared by selfassembly of polyethylene glycol and poly. Pgabased nanoparticles used as a vector for effective and safe anticancer therapy, owing to their controlled and sustainedrelease properties, low toxicity, as well as biocompatibility with tissue and cells. A facile and green approach is reported to construct pixantrone poly glutamic acid nanoparticles pixpga nps as an oral drug delivery system through the complex self. Nanoparticles and hydrogels have gained notable attention as promising potential for fabrication of scaffolds and delivering materials. Here, we report a metabolically engineered corynebacterium glutamicum capable of producing. To develop a safe and effective mucosal vaccine that broad cross protection against seasonal or emerging influenza a viruses, we generated a mucosal influenza vaccine system combining the highly conserved matrix protein2 sm2, fusion peptide of. The researchers are focused on making potential efforts for the development of nanoparticles based on biodegradable polymer poly gamma glutamic acid.
Pga is considered a promising biobased chemical and is already widely used in the food, medical, and wastewater industries due to its biodegradable, nontoxic, and. The care solution with poly gamma glutamic acid showed better properties on cytotoxicity, irritation, and lubrication. Pdf nanoparticles prepared by selfassembly of chitosan. Preparation of nanoparticles composed of chitosan poly gamma glutamic acid and evaluation of their permeability through caco2 cells. However, the relation between proline metabolism and. Pga is a microbesecreted isopeptide that has been shown to promote growth and enhance stress tolerance in crops. Optimal skin hydration is a critical factor to beautiful and healthy skin. Nanoparticles prepared by selfassembly of chitosan and. Nanoparticles prepared by selfassembly of chitosan and poly. Although single administration with jevlp protected less than 50% of mice against lethal jev infection, adding poly.
Pdf mucosal vaccination of conserved sm2, ha2 and cholera. In this study, a hydrophilic and nontoxic biopolymer poly. Polygammaglutamic acid nanoparticles combined with mucosal influenza virus hemagglutinin vaccine protects against influenza virus infection in mice. In 2009, the global outbreak of an influenza pandemic emphasized the need for an effective vaccine adjuvant.
Pga nanoparticles nps to modulate macrophages and dcs inflammatory profile and to impair their ability to promote cancer cell invasion. Gammapolyglutamic acid gammapga is a natural occurring, multifunctional, and biodegradable biopolymer. The present composite nanoparticles were prepared with three biocompatible polymers of chitosan, poly glutamic acid, and pluronic using a simple ionic gelation technology. It has been shown that lower friction coefficients are correlated with increased comfort. Ha vaccine with gamma pganps protected mice against challenges. Hydrolytic and enzymatic degradation of nanoparticles. Owing to its biodegradable, nontoxic and nonimmunogenic properties, it has been used successfully in the food, medical and wastewater industries. Antigen delivery systems using polymeric nanoparticles are of special interest as stable protein. In this study nanogel was created from methacrylated poly gamma glutamic acid nanoparticles by visible dental blue light photopolymerization. Pga is a biodegradable polymer naturally produced by bacillus spp. Polyglutamic acid nanoparticles combined with mucosal influenza virus hemagglutinin vaccine protects against influenza virus infection in mice article pdf available in vaccine 2742. Pga can exhibit different properties conformational states, enantiomeric properties and molecular mass.
Pga and the anticancer drug pixantrone dimaleate pdm. The present paper describes the preparation and characterization of novel biodegradable nanoparticles based on selfassembly of poly. Pga composite dressing was prepared for biomedical applications. Pga is a naturally occurring biopolymer made up of repeating units of l glutamic acid, d glutamic acid or both. Glutamic acid often is used as a food additive and flavor enhancer in the form of its sodium salt, known as monosodium glutamate msg. It is a homopolyamide, composed of glutamic acid monomers connected by amide linkages between. To address this problem, we developed a fully insertable poly glutamic acid microneedles with a supporting structure design to ensure complete and efficient delivery of encapsulated drugs. Pganps, a safe, natural material, to subcutaneous immunization with influenza virus hemagglutinin ha vaccine increases the protective immune responses against influenza virus in mice. In this study, a novel nanoparticle system for paracellular transport was prepared using a simple and mild ionicgelation method upon addition of a poly. Pdf safety and photochemotherapeutic application of poly. The correlation of size of particles, ph of the solutions, concentration, order of. Pga is an anionic polyamide with numerous applications. The present invention relates to an immunoadjuvant composition containing polygammaglutamic acid chitosan nanoparticles and a composition for vaccine containing the immunoadjuvant, and more particularly, to nanoparticles by ion bonding of polygammaglutamic acid and chitosan, which have secured safety.
Composition for adjuvant comprising poly gamma glutamic acid chitosan nanoparticles. Some proteinrich plant foods also serve as sources. It is produced through fermentation by bacillus subtilis using glutamic acid. The particle size and the zeta potential value of the prepared nanoparticles can be controlled by their constituted compositions. Pdf pharmaceutical and medical applications of poly. Sustainable release of paclitaxel from biodegradable poly gamma. Pga solution into a lowmolecularweight chitosan lowmw cs solution. We have previously developed a multipurpose contact lens care solution in which polygammaglutamic acid. Poly glutamic acid nanoparticles and lowintensity ultrasound. Volume 25, issue 49, 28 november 2007, pages 82708278. Preparation of nanoparticles composed of chitosanpoly.
Poly glutamic acid nanoparticles combined with mucosal influenza virus hemagglutinin vaccine protects against influenza virus infection in mice article pdf available in vaccine 2742. Poly gamma glutamic acid nanoparticles combined with mucosal influenza virus hemagglutinin vaccine protects against influenza virus infection in mice. Therefore, designing an excellent antibacterial hydrogel with a suitable wateradsorbing capacity is very important to improve the development of wound dressings. Polygammaglutamic acid nanoparticles combined with mucosal influenza virus hemagglutinin vaccine protects against influenza virus infection in. Antiinflammatory chitosan poly gamma glutamic acid nanoparticles control inflammation while remodeling extracellular matrix in degenerated intervertebral disc. We report a curcumin nanoparticle with a tripolymeric composite that can be used as a delivery device for wound healing. In the present work, we assessed the potential of chitosan ch poly.
The nanoparticles were prepared by a precipitation method, and about 200 nmsized nanoparticles were obtained due to their amphiphilic properties. In addition, comfort is an important issue for contact lens wearers due to the long periods of time they are worn. However, the therapeutic effects of cdt are hindered by low reaction speeds. Attention has been drawn to pga because it is biodegradable, biocompatible, nontoxic and nonimmunogenic. Bacterialderived polymer polyglutamic acid pgabased. Pga nanofibers nfs are first fabricated by solution blowing with the help of polylactic acid pla and designed to form amino acid arrays as. Pga nps as an oral drug delivery system through the complex selfassembly of polyelectrolyte. The resulting poly gamma glutamic acid derivatives in the form of finely controlled hydrophilicity are provided.
Relation between gelation conditions and the physical properties of whey protein particles. The care solution with poly gamma glutamic acid showed better properties. The present paper describes the preparation and characterization of novel biodegradable nanoparticles based on selfassembly of poly gamma glutamic acid. Preliminary investigation on a new natural based poly. Pga designed nanoparticle drug delivery systems, which are used in many.
In this paper, a novel silver nanoparticles poly gamma glutamic acid. All meats, poultry, fish, eggs, dairy products, and kombu are excellent sources of glutamic acid. Pdf sustainable release of paclitaxel from biodegradable. Gamma poly glutamic acid has been implicated in epileptic seizures. The nanosystems were stable inaqueous media at low ph conditions.
Significant research efforts have been focused on the development of biodegradable polymer poly gamma glutamic acid. Mar 26, 2009 for the purpose of targeted drug delivery, composite biodegradable nanoparticles were prepared from chitosan and the poly. This gene encodes a vitamin kdependent, gamma carboxyglutamic acid glacontaining, singlepass transmembrane protein. The average size of the particles was 80 nm by dls, and the nmr spectra showed that the methacrylation rate was 10%. Glutamic acid promotes enhanced dechlorination of p. Structural and dynamical characterization of poly gamma glutamic acid based crosslinked nanoparticles article pdf available in colloid and polymer science 2864. One of the most recently discovered biopolymers for the application of nanoparticles is poly gamma glutamic acid pga. Aquamax represents a new class of naturebased, functional moisturizers that not only deliver improved moisturization to the skin through their excellent humectant properties, research suggests that aquamax may also affect a number of biological processes in the skin that can contribute to the maintenance of. Pga is a naturally occurring biopolymer made up of repeating units of glutamic acid and can be potentially used for multiple applications. Kr1042641b1 composition for adjuvant comprising poly.
Pga was found to increase levels of nk cellmediated cytotoxicity and inf. Incomplete insertion of microneedles largely limits drug delivery efficiency and wastage of valuable medication. A facile and green approach is reported to construct pixantrone poly. Pga using bacillus species often requires the supplementation of l glutamic acid, which greatly increases the overall cost. The introduction of cholesterol into poly gamma glutamic acid pga provides a pga derivative in particulate form having a particle size of 501,000 nm. Chitosan cs 25 is a cationic polysaccharide derived from chitin that is widely used in food and pharmaceutical industries as well as in other fields. Gamma polyglutamic acid gamma pga is a natural occurring, multifunctional, and biodegradable biopolymer. In the present study, novel biodegradable poly glutamic acid pga nanoparticles were examined for their antigen delivery and immunostimulatory activities in vitro and in vivo.
Solubility of the systems was determined by turbidity measurements. Oct 08, 2018 significant research efforts have been focused on the development of biodegradable polymer polygammaglutamic acid. Pga is produced by fermentation in bacillus species. Structural and dynamical characterization of polygamma. Pga 23,24 is a natural anionic polymer material that shows good cell affinity, is nontoxic and harmless, and is degradable. Pharmaceutical and medical applications of poly gamma glutamic acid 5 sung et al. Visible lightcurable systems can allow for the possibility of in situ fabrication and have the advantage of optimal applicability. Pga is a type of polyamide composed of single glutamic acids joined via.1539 825 1208 617 166 528 1585 1421 890 485 1433 1460 26 474 1326 246 436 510 534 1360 719 1341 318 570 146 964 404